Antibodies to HIV (e.g., EIA, rapid test, Western blot. Any duplication or distribution of the information contained herein is strictly prohibited. ing, for example, HIV surveillance in populations not accessible through clinic settings (e.g. Before, the proteins were separated according to thei. Links to other sites are provided for information only-they do not constitute endorsements of those other sites. Hey Friends,Western Blot is a technique to determine whether a specific protein is present in a sample. A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions. is also a founding member of Hi-Ethics and subscribes to the principles of the Health on the Net Foundation (The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. Learn more about A.D.A.M.'s editorial policy, editorial process and privacy policy. is among the first to achieve this important distinction for online health information and services. follows rigorous standards of quality and accountability. is accredited by URAC, also known as the American Accreditation HealthCare Commission (URAC's accreditation program is an independent audit to verify that A.D.A.M. If the results are indeterminate, the test may need to be repeated after about a month.Ī.D.A.M., Inc. A positive ELISA indicates the presence of antibodies to a virus in the sample. Timing and frequency of lab tests may vary if they are performed for professional or legal reasons. A positive Western Blot indicates the presence of viral antigen which very often means live virus in the sample. Certain tests may be repeated to obtain a series of results, or tests may need to be repeated to confirm or disprove results. Conditions that worsen and improve may also need frequent monitoring. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. The age or gender of the person being tested may affect when and how often a lab test is required. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. A test may be suggested or become necessary when certain signs or symptoms appear.ĭue to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. However, analysis of the protein can be difficult if multiple bands appear on the blot. When and how often laboratory tests are done may depend on many factors. The Western blot assay provides valuable information about a protein including abundance, the apparent molecular mass, post-translational modifications and splice variants.
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